关键词:酶催化氧化;处理;己烯雌酚
Enzyme-Catalyzed Oxidation of Diethylibestrol in Aqueous Phase
LI Hong-mei
(School of Safety and Environmental Engineering, Capital University of Economics and Business, Beijing 100070, China)
Key words: enzyme-catalyzed oxidation; disposal; diethylibestrol
1.1 仪器与试剂
Britton-Robinson缓冲溶液配制方法参考文献[23]。
HRP活性测定采用ABTS法[25]。
2 结果与分析
2.1 pH对DES去除效果的影响
2.3 HRP与DES用量之比对DES去除率的影晌
2.4 H2O2浓度对DES去除率的影晌
2.5 DES起始浓度对去除速率的影晌
3 结论
试验得出以下结论:
2)HRP催化H2O2氧化DES的适宜温度为25 ℃。
3)DES去除率随[HRP]0/[DES]0增大而升高。当[HRP]0/[DES]0大于0.5 U/μmol时, DES去除率超过90%。[HRP]0/[DES]0的适宜比值为1.000 U/μmol。
5)DES起始浓度越高,其去除速率越大。以底物DES表示的最大反应速率Vmax和米氏常数Km分别为74.63 mmol/(L・h)和46.75 mmol/L。
参考文献:
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